更多“简述3’-RACE。”相关问题
  • 第1题:

    Fisher-Race提出的CDE命令法认为红细胞Rh基因是几个基因的复合物

    A.1

    B.2

    C.3

    D.4


    本题答案:C
    本题解析:Fisher-Race提出的CDE命令法认为Rh基因是3个基因的复合物,每条染色体上有3个基因位点相互连锁;每个基因决定1个抗原;基因和基因产物使用相同的名称。

  • 第2题:

    ___ variables are factors like age, gender, ethnic background, race, religion, education and income.

    A.Geographic

    B.Demographic

    C.Psychographic

    D.Product-use


    正确答案:B

  • 第3题:

    The following day I ran my first race at high__________.

    A. latitude
    B. altitude
    C. longitude
    D. attitude

    答案:B
    解析:
    考查名词辨析。latitude意为“纬度,选择的余地”,altitude意为“高度,海拔高度”,longitude意为“经度”,attitude意为“态度;看法”。根据句意“第二天我第一次在高海拔地区参加了赛跑”可知选B。

  • 第4题:

    The famous athlete expressed his regret at the failure of the running race.

    A: disapproval
    B: sorry
    C: shame
    D: disagree

    答案:B
    解析:
    句意为:那位知名运动员为自己比赛的失利表示遗憾。 regret意为“后悔、遗憾”;dis-approval意为“反对、不赞成”; sorry意为“遗憾”; shame意为“羞辱、丢脸”; disagree意为“不同意”。

  • 第5题:

    Fisher-Race提出的CDE命令法认为红细胞Rh基因是几个基因的复合物

    • A、1
    • B、2
    • C、3
    • D、4

    正确答案:C

  • 第6题:

    非小种专化抗病性(race-nonspecific resistance)


    正确答案: 具有该种抗病性的寄主品种与病原物小种间无明显特异性相互作用,是由微效基因控制的,针对病原物整个群体的一类抗病性。

  • 第7题:

    小种(race)


    正确答案: 病原物“种”下的一个分类术语通常指病原物不同菌株间在一组鉴别品种上的致病力有专化性分化的类群,它们在形态、生化性状上都无明显差异。

  • 第8题:

    RACE


    正确答案:是一种通过PCR进行cDNA末端快速克隆的技术,是以mRNA为模板反转录成cDNA第一链后用PCR技术扩增出某个特异位点到3,或5,端之间未知序列的方法。

  • 第9题:

    问答题
    简述3’-RACE。

    正确答案: 3’RACE用于mRNA已知序列下游(即3’端)未知序列的扩增,可分为两步。
    (1)以与polyA尾巴互补的序列为引物, 反转录获得3’末端第一链cDNA序列。
    (2)以靠近第一链cDNA3’端的序列为特异引物合成cDNA第二链,再PCR扩增。
    A.反转录获得3’末端第一链cDNA:3’末端本身具有PolyA,可以与引物结合。引物也同样具有oligo(dT),但这种引物在5’端有OP(Outer Primer)和IP(Inner Primer)的特殊结构,便于为以后的两轮PCR提供引物;在3’端加入一个锚定核苷酸,可以使反转录在紧接着PolyA的交界处进行,这样可以有效减少同聚物的产生。
    B.第一链cDNA3’端的扩增:设计两种引物——GSOP和GSIP。由于cDNA已含有外源性OP和IP序列,故可以用来自目的基因的GSOP和来自锚定序列的外源性OP进行第一轮扩增,用GSIP和IP进行第二轮扩增,以此增强特异性。
    解析: 暂无解析

  • 第10题:

    名词解释题
    小种(race)

    正确答案: 病原物“种”下的一个分类术语通常指病原物不同菌株间在一组鉴别品种上的致病力有专化性分化的类群,它们在形态、生化性状上都无明显差异。
    解析: 暂无解析

  • 第11题:

    单选题
    A

    Habitual criminals.

    B

    Crime’s relationship to race.

    C

    Prisoners by gender and race.

    D

    Crime rates and prisons.


    正确答案: D
    解析:
    教授首先对三种监狱进行了讲解,之后又讲了由于年代、种族和性别等的不同,犯罪率存在差异。

  • 第12题:

    The sentiment that justifies cruelty to beasts leads to persecution of men of other race, class or religion.()


    参考答案:正确

  • 第13题:

    Life is like a long race_____we compete with others to go beyond ourselves.

    A. why B. what C. that D. where


    正确答案:D

  • 第14题:

    The law does not discriminate( )any race,creed and color.
    against


    答案:
    解析:
    discriminate against“歧视”。句意:法律不能因为人种、信仰和肤色的不同而有区别。discriminate的他搭配:discriminate between区别……与……,discriminate in favor of对……特别好些。

  • 第15题:

    I stroll around the park for an hour after dinner every day.

    A:run
    B:roll
    C:walk
    D:race

    答案:C
    解析:
    stroll:漫步,散步。题干意思为:我每天晚饭之后都在公园漫步一小时。 run:跑步;roll:滚动;walk:步行,散步;race:比赛,赛跑。经过分析,C选项最为合适。

  • 第16题:

    The earliest invasion is that by the ()-haired Mediterranean race called the Iberians.


    正确答案:dark

  • 第17题:

    小种专化抗病性(race-specific resistance)


    正确答案: 对专性寄生物或部分兼性寄生物,寄主的抗病性可以仅仅针对病原物群体中的少数几个特定小种,称为小种专化抗病性。

  • 第18题:

    试述5′RACE技术原理和方法。


    正确答案: PCR用于扩增代表mRNA转录物某一单位点与其3′或5′末端之间区域的部分cDNA称为快速扩增cDNA末端技术(RACE)。如果已知mRNA的一片段链内短序列,据此或设计基因特异引物,用原先存在的poly(A)尾(3′末端)或附加的同聚尾(5′末端)互补的序列做末端引物,就可以获得从未知末端直到已知区域的部分cDNA序列。为获得5′末端部分cDNA克隆需用基因特异引物,产物第一链产物,可用末端脱氧核苷酸转移酶及dATP加poly(A)尾。通过QT引物和反转录使用的上游基因特异引物生成第二链cDNA。

  • 第19题:

    单选题
    Fisher-Race提出的CDE命令法认为红细胞Rh基因是几个基因的复合物
    A

    1

    B

    2

    C

    3

    D

    4


    正确答案: C
    解析: Fisher-Race提出的CDE命令法认为Rh基因是3个基因的复合物,每条染色体上有3个基因位点相互连锁;每个基因决定1个抗原;基因和基因产物使用相同的名称。

  • 第20题:

    名词解释题
    RACE

    正确答案: 是一种通过PCR进行cDNA末端快速克隆的技术,是以mRNA为模板反转录成cDNA第一链后用PCR技术扩增出某个特异位点到3,或5,端之间未知序列的方法。
    解析: 暂无解析

  • 第21题:

    单选题
    At 0630 you increase speed to 12.0 knots. At 0645 Race Rock Light bears 294°pgc. At 0700 Race Rock Light bears 293°pgc. Based on this,you should().
    A

    alter course to port

    B

    maintain course and speed

    C

    alter course to starboard

    D

    maintain course and reduce speed


    正确答案: B
    解析: 暂无解析

  • 第22题:

    单选题
    300 Hurdles Now comes the first hurdle. __1__ We all make it over this hurdle with ease. This symbolizes. Those obstacles in life that we struggle to get over, but we always manage to do so. You can look at that hurdle in two ways: "I can’t make it over, I’m too tired" or "I am one more hurdle closer to finishing this race". This is an example of taking a negative situation and seeing something positive in it. The next part of the race is every hurdle between the first and last one. __2__ This happens a lot in life too. There may be a situation that arises where you feel as though you can’t make it and all you can think about is giving up. You may fall over that "hurdle" or struggle getting over it, but somehow you find it in yourself to jump over it and move on with the race. Next is that last 100m of the race. You feel as though you just want to say, "No I can't do this." __3__. Now lastly is the last hurdle. This hurdle is the toughest out of all of them. In life we find ourselves falling and falling, and maybe getting too lost in our problems that we fell like have been though enough and there’s no hope. __4__ It's one step close to the end of the race. Once you finish, you look back on it and you realize that you made it not by leaping, but step by step. "Yard by yard, life is very hard. But inch by inch, life can be a cinch."__5__. __3__内应选()
    A

    This hurdle the easiest to get over because you are not worn out from running.

    B

    Again, you must find it in yourself that you can do it, and you must finish the race stronger than when you had started it.

    C

    All it takes is one step at a time, one hurdle at a time.

    D

    That last hurdle symbolizes these situations.

    E

    These hurdles get tougher and tougher and you may feel as though you can’t go on.

    F

    Next is the lining up in our own individual lanes.


    正确答案: B
    解析: 上文提到当我们来到最后100米跨栏时,我们想说“不,我做不到。”与上段一样,作者先提出困难然后指出应该克服困难。因此应填入表示克服困难的句子。选项B中的you can do it... you must finish the race…符合此意,并与上句的“I can't do this”相对应。答案为B。